Engineering a Dual Small Molecule Gated ZAP70 Switch in T Cells
نویسندگان
چکیده
منابع مشابه
Regulatory T cells Suppress Zap70 Phosphorylation in Responding T cells
Objective: Regulatory T cells (Treg) have a critical role in controlling responding T cells (Tresp), thereby preventing autoimmune manifestations including those observed in adenosine deaminase (ADA) enzyme-deficient patients and mice (ADA-KO). Treg suppress various functions of Tresp such as proliferation, cytokine production and expression of activation markers, yet it is not known if Treg su...
متن کاملRemote control of therapeutic T cells through a small molecule-gated chimeric receptor.
There is growing interest in using engineered cells as therapeutic agents. For example, synthetic chimeric antigen receptors (CARs) can redirect T cells to recognize and eliminate tumor cells expressing specific antigens. Despite promising clinical results, these engineered T cells can exhibit excessive activity that is difficult to control and can cause severe toxicity. We designed "ON-switch"...
متن کاملConformationally gated electron transfer in iso-1-cytochrome c: engineering the rate of a conformational switch.
An engineered form of iso-1-cytochrome c with lysine 73 mutated to histidine is shown to increase by nearly 500-fold the rate of a conformational gate that modulates the rate of electron transfer into this protein. This result demonstrates the potential of protein engineering to provide electron transfer gates with tailored properties. The pH dependence of the rate of the conformational electro...
متن کاملMetabolic engineering--a genetic toolbox for small molecule organic synthesis.
Metabolic engineering is an emerging field that exploits biosynthetic machinery as a means to genetically design small molecule production within heterologous host organisms. Molecular design and synthesis with biological tools has lagged behind total synthesis technology for about seventy-five years and owes its existence to relatively new molecular biology techniques. Here the field of metabo...
متن کاملEffects of GW9508 small molecule on oxidative stress enzymes in colorectal cancer and non-cancerous HUVEC cells
Background: In this study, we aimed to evaluate the effects of GW9508 as an unsaturated fatty acid on inducing oxidative stress in HT-29 cancer cell line and non-cancer HUVEC cells. Materials and methods: The effects of GW9508 on cell viability were determined by performing MTT assay after 1, 3 and 5 days. The anti-oxidant superoxide dismutase (SOD) and catalase enzymes' assay was performed to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Synthetic Biology
سال: 2018
ISSN: 2161-5063,2161-5063
DOI: 10.1021/acssynbio.7b00394